بررسي اثرات قطع طناب نخاعي بر ساختار هيستومتر فومتريك تخمدان رت

Effect of spinal cord injury on ovarian histomorphometric structure in rats

One of the important disorders subsequent to spinal cord injury is the sexual disorders at both sexes . This can be the source of psychic and mental problems for patients . The purpose of the present study is to investigate histological and morphological changes of ovary at the acute phase after spinal cord injury . Adult female Wistar rats divided two groups (Experimental =EXP and control =CON groups) each group contain 24 rats. The spinal cord of EXP animals transected by bilateral laminectomy on level T9. The animals of both groups were killed at 7th , 14th and 21st days from operation and their weight , the weight and volume of ovaries were measured . The specimens serially sectioned in 4 micrometre and the slices were stained by the H&E , PAS and Trichrome masson methods. The quantitative and qualitative of samples changes were studied by light microscope equipped with eye piece and statistically analyzed using t-test and ANOVA. The results show that the animal weight, the volume and weight of ovaries decreased at three EXP groups (p<0.05) . In the EXP groups the diameter of follicle and ovum and the thickness of granulosa layer decreased significantly in different kinds of follicles ( Multilaminar primary follicle = MPF, Primary antral follicle = PAF , Secondary antral follicle = SAF and Graafian follicle = GF) (P<0.05) . Also the thickness of zona pellocida decreased at PAF . SAF and GF in EXP groups (P<0.05) . The thickness of theca internal of SAF and GF decreased significantly in the groups of 14th and 21 th days and (P<0.05). The ovarian stroma showed Edema , Fibrosis , Hypercellularity and Vasodilation . These findings suggest that spinal cord injury induced histological changes and hetrogenic increase at ovarian structure. This probably results from the lack of an autonomic function and the modification at the neurotransmiters.