اثر هورمون هاي گياهي بر جوانه زايي مستقيم از جداكشت هاي گياه چغندرقند

Investigating the Effect of Plant Hormones on Direct Shoot Regeneration from Sugar Beet Explants

In order to investigate rapid and direct shoot regeneration, various explants of sugar beet were statistically tested. In the study, shoot tip, hypocotyl, cotyledon petiole, leaf petiole, leaves of seed- grown seedling and leaves of tissue cultured - shoot tip explants were used. Shoot tip explants of 10-day old seedlings produced 66% shoot in the medium containing 2 mg/I Benzyl Adenin (BA), 0.5 mg/l Naphtalen Acetic Acid (NAA) and 0.5mg/l Tri Ido Benzoic Acid (T1BA). A high rate (55%) of shoot regeneration was obtained from leaf petioles of 25-day old seedlings in the medium containing I mg/l BA and 1 mg/l NAA. In addition, leaves originated from tissue cultured shoot tips regenerated 42 shoots in total in the medium containing 1mg/l BA and 1mg/l NAA. No shoot was regenerated from hypocotyl, cotyledon petiole and seed - grown seedling leaves in the examined hormonal treatments with BA, NAA, TIBA and a combination of these hormones. The results suggest that shoot tips, leaf petioles and leaves of tissue - cultured - shoot tips in the media containing appropriate concentrations of BA, NAA and TIBA can be used for direct shoot regeneration with a frequency sufficient for fulfilling an important requirement for gene transformation in sugar beet.