انگيزش و رشد پنبه در ارقام رزمينياتور

CALLUS INDUCTION AND GROWTH IN MINIATURE ROSE (ROSA CHINENSIS JA.CQ. VAR. MINIMA REHD) CULTIVARS

Several experiments were conducted for selecting the optimum callus induction and growth conditions in three miniature rose cultivars (ʹLittle Buckarooʹ, ʹBaby Masqueradeʹ and ʹSouratiʹ). In preliminary experiments, leaflet explants were found the best among different explants and were cultured on MS medium supplemented with benzyl adenine (BA) and indoleacetic acid (IAA) or indolebutyric acid (IBA) or naphthalene acetic acid (NAA). Explants that were cultured on MS medium supplemented with 0.5 mg 1-1 BA and 2 mg 1-1 NAA, had the best callus production in establishment and further subculture media. Forthy days after the frist subculture, ʹBaby Masqueradeʹ and ʹSouratiʹ, had the highest and lowest callus growth, respectively. Different treatments such as using casein hydrolysate, yeast extract, red light and changing the light intensity were applied which were unsuccessful for shoot and/or root regeneration in calli.