پديد آورندگان :
مرتضي خوشخوي ، مترجم , , شرف زاده ، شهرام نويسنده ,
كليدواژه :
Tissue culture , افزايش درون شيشه اي , ريزافزايي , كشت بافت , استهبان , In vitro propagation , كشاورزي , Micropropagation , SAFFRON , زغفران
چكيده لاتين :
Saffron is one of the native plants of Iran. In this investigation, suitable culture medium and growth regulator requirements for in vitro propagation of saffron were studied. Corms of `Estahbanʹ saffron were planted for tissue culture studies at College of Agriculture, Shiraz University. Half of corms were kept at 4-5 °C and the others at 15-30 °C. Half-strength Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1 and 1.5 mg 1^-1 2,4-D and BA, 500 mg 1^-1 casein hydrolysate and 50 g 1^-1 sucrose was used for cormlet production. In vitro grown cormlets were transferred to MS medium with 1.4, 1.6, 1.8, 2.0, 2.2, 2.4, 2.6. 2.8 and 3.0 rng 1^-1 NAA for further growth. A half-strength MS medium supplemented with 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 mg 1^-1 BA and NAA was used for shoot production experiments. Number of in vitro produced cormlets, cormlet diameter and shoot length were recorded after 3, 6 and 8 weeks, respectively. Results indicated that low temperature pretreatment is necessary for in vitro cormlet production and larger corms produced more cormlets. 0.5 mg 1^-1 BA and 1 mg 1^-1 2,4-D were optimum concentrations for cormlet production. Similarly, 1.8 mg 1^-1 NAA for cormlet growth and 10 mg 1^-1 NAA and 10 mg 1^-1 BA for shoot production were the optimum concentrations.
