بررسي تنوع ژنتيكي توده هاي وحشي بادام استان اصفهان با استفاده از برخي ويژگيهاي مورفولوژيك و پروتيين هاي ذخيره اي بذر

GENETIC DIVERSITY AMONG WILD POPULATIONS OF ALMOND (AMYGDALUS SPP.) IN ISFAHAN PROVINCE AS DETERMINED BY SOME MORPHOLOGICAL CHARACTERISTICS AND SEED STORAGE PROTEINS

Forty wild almond trees were sampled and studied using morphological traits and protein markers in each of the following areas in Isfahan province: Pashandegan, Mahorestan, Vanak. 1-Kashan. Naien and 2-Kashan in 2001. Morphological traits were included leaf length, leaf width, petiole length, leaf area, shell and kernel dimensions and shell and kernel weigth .The variability of seed storage proteins were analyzed electrophoretically and eight bands were observed, attributed to four loci: A (two alleles), B (three alleles), C (two alleles) and D (as a complex locus). Three species of Amygdales haussknechtii (Pashandegan, Mahorestan and Vanak populations), A. scoparia (I-Kashan and Naien populations) and A.lycioides (2-Kashan population) were detected. Cluster analysis based on squared euclidean distance in morphological traits and Nei genetic distance in protein markers related to SDS-PAGE using UPGMA method indicated the same and clear grouping. Thus, protein markers can be used even more efficiently instead of evaluation of morphological traits which is more cost and time consuming.