با درجه خلوص پزشكي (1) B21 بررسي طريقه توليد راديو داروي ويتامين

Investigation for Preparation and Production of Radio-Kit Vitamin B12 with Med-grade (Part-1)

Labeled vitamin B12 with 58 Co is one of the important ingredients of the diagnostic Kits for diagnosis of patients affected by anemia. The insufficient absorption of vitamin B12 is one of the causes of anemia in humans and one of the accurate methods for measurement of the absorbed vitamin B^ is application of labeled vitamin B12 with the cobalt-58 radioisotope. The isolation and purifications of the labeled vitamin B12 from fermentation medium of Streptomyces Olivaceus is an essential process for kit preparation. The first experiment in this research was isolation and purification of B12 using different resins with the solution of cyanocobalamin containing cobalt-59, produced by this bacterium. After investigation and pre-feasibility experiments two non-polar resins XAD-4 and XAD-7 were selected. The results that obtained from different experiments on XAD-4 and XAD-7 showed that XAD-4 is much better than the latter one due to immobilization of cobalamin. The isolation of vitamin B12 on XAD-4 column was achieved by different solvents such as methanol, ethanol and isopropanol in different concentrations ratios. The results of TLC (Thin Layer Chromatography) showed that the best eluant solutions for desorbtion of cobalamin from XAD- 4 column are solvents such as methanol, ethanol and isopropanol with the maximum concentration of vitamin B12 in the concentration ranges of 25 up to 50 percent, 30 up to 40 per-cent and 10 per-cent, respectively. Cobalamin was collected in acetone and crystallized in low temperature.