تاثير تجويز مقادير مختلف استرپتوزوتوسين بر پارامترهاي مورفومتريك كليه رت

Effect of administration of different doses of streptozotocin on renal morphometric parameters in rats

Introduction: Due to the relationship between structure and function, the study of glomerular volume is important to understand initiation and progression of diabetic glomerulopathy. The aims of the present study were to estimate renal volume as well as absolute glomerular volume and total glomerular number in diabetic rats following administration of different doses of streptozotocin (STZ), by using unbiased morphometric methods. Material and Methods: Male Wistar rats with an average weight of 110 gr were randomly divided into 5 groups. Diabetes was Induced by an IP injection of Stereptozotocln 15mglkg, 45mg/kg and 90 mg/kg. Fifty-six days: after 1P Injection, the animals were anesthetized by ketamine hydrochloride and the kidneys were excised, decapsulated and fixed in modified Lilliʹs solution. After tissue processing and staining, new morphometric methods were used to estimate the volumes of different zones of kidney, absolute glomerular volume and total glomerular number. Results: This experiment showed increased cortical tissue volume in all groups whereas medullary volume increased only in the group which received 90mg/kg of STZ (P<0.01). Glomerular volume was increased significantly in all groups (P<0.01) but no changes in total glomerular number were detected (P>0.05). Conclusion: This study revealed that, at least, after short periods of inducing diabetes mellitus, administration of more STZ might have more effects on kidneys and cause more imbalances In cells turnover.