مطالعات آنزيمي در ارتباط با چوبي شدن در اكاليپتوس

Enzymatic Studies as Related to Wood Production in Eucalyptus Camaldulensis Dehn

To make a primary investigation of lignin content variation as related to content of peroxidase enzyme in different seasons and in different sites, samples were taken in four regions, and during two seasons, of eucalyptus (Eucalyptus camaldulensis) trees. Qualitative and quantitative survey was done by employing PAGE and spectrophotometry methods. The Content of Kiason lignin was determined using extractive free wood sawdust. The results show that peroxidase isoenzyme patterns change depending upon region and season. This can be speculated as a part of tree physiological change, preparing to resist against cold. It was also observed that there are differences among ecological regions as well as among trees in the same region. Based upon the genetics of the trees, lignin content in Khuzistan region decreases in winter and so does enzyme activity. Lignin content was directly related to branch diameter as well as enzyme activity.