RAPD عامل بيماري پوسيدگي طوقه برنج با استفاده از ماركرهاي مولكولي FUSARIUM MONILIFORME شناسايي جدايه هاي قارچ

Identification of Isolates of Fusarium moniliforme, the Causal Agent of Rice Foot Rot Disease, Using RAPD Technique

In this study 58 isolates of Fusarium moniliforme which causes rice foot rot disease were collected from infected rice seeds. The morphological characteristics and pathogenicity of these isolates were determined in laboratory and greenhouse in the international Rice Research Institute (IRRI). Besides, by RAPD (Random Amplified Polymorphic DNA) technique using primer .T-l1 from operon kit by sequences of "5-ACTCCTGCGA-3" the randomized portion of the DNA of each isolate was amplified. Using cluster analysis at 25% similarities, the isolates were classified as follows: from point of the growth rate of fungi after 4 days in Petri dishes into 8 groups; from point of the number of days that fungi reached to the 90 mm diameter in one group; from point of the effect of isolates on the length of seedlings into 5 groups; from point of the genetical pattern into 13 groups. The characteristics measured in the laboratory or greehouse for each isolate did not significantly differ with the mean of each group using t-test analysis. Therefore, it could be concluded that molecular markers by RAPD technique can be used in differentiation of morpological and pathogenicity of Fusarium moniliforme isolates. The positive significant correlation coefficient (r = 0.54) was observed between the growth rate of fungi in Petri dishes and its effect on the length of seedling.