بهينه سازي استخراج كيتين و تهيه كيتوسان از پوست ميگو: بررسي عوامل موثر برواكشن استيل زدايي كيتين و ارزيابي زيست سازگاري

Optimization of Chitin Extraction from Shrimpʹs Shell and Chitosan Preparation: Study of Parameters Influence on Chitin Deacetylation Reaction and Bioeompatihility Evaluation (In vitro)

In chitin extraction from the shrimpʹs shell the influence of extraction parameters (reaction time length, temperature and concentration of reagents) was studied. In this study chitin was extracted from shrimps shell of Persian Gulf, using different methods. The process parameters were optimized to chitin with 6.8% nitrogen content and the viscosity average molecular weight (Mv) of 3.22* 10^6 and ash content of 0.34. Chitin extraction yield from shrimpʹs shell was 25-30%. Degree of deacetylation (DD) and molecular weight are structural parameters which influence industrial uses such as medical application. Deacetylation of chitin was carried out by optimized reaction parameters (reaction time length, temperature, concentration of alkaline reagent and sea sources of chitin). Experimental deacetylation conditions were studied and plnsicochemical characteristics of the obtained chitosan were compared. This work suggests that at these experimental deacetylation conditions (T=90. t=5h. freshness of chitin and 50% NAOH) it is possible tO reach molecular weight as high as 3.57 * 10" and degree of deacetylation of 86%.