تخليص و جداسازي سلولهاي جزاير لانگرهانس موش صحرايي

Isolation and purification of rat islet cells

Background and Purpose: It is thought that transplantation of islet cells would cure diabetic patients in world. Islet cells transplantation in people suffering from diabetes is of technical interest. A standardized procedure was developed for the preparation of rat islet cell grafts for purification of islet cells. Materials and methods: In this process, after collagens digestion of pancreases, islets were isolated and dissociated, then with enzymatic procedure by DNase and trypsin, the islet cells changed in to single cells and these cells were assayed by flow cytometery. Results: Flow cytometery of these cells indicated that there were 91% of beta cells in cell suspension. Most of the exocrine particles were lost during digestion. Conclusion: Purified endocrine islet cell grafts were prepared by pure beta-cells, with or without endocrine non-beta cells. The purified aggregates were devoid of non-endocrine cells and damaged cells.