كليدواژه :
پراكسيداز , ليپيد , شوري , كشت بافت , پروتيين , پنبه , پلي فنل اكسيداز
چكيده لاتين :
The effects of different concentration of NaC! (0,25, 50, 100, 200, 400 mM) on growth of dissected root embryos of cotton (Gossypium hirsutum L). C.V. Sahel total protein and lipid content, activity of peroxidase and polyphenoloxidase after four weeks of culture on MS medium were studied. MS medium contained IAA (5mg/L), BA (0.25mg/L) and different concentration of NaCl. The growth rate was 100% in all treatments and the highest growth rates of embryo and hypocotyl were 0-100 and 0 - 50 mM of NaCl respectively. Highest rhizogenesis were observed with 50 and 25 mM after 2 and 4 weeks, respectively. Growth of cotyledons at 500 mM NaCl as well as apical bud, and initiation of leaves in medium with 25 mM NaCl have been occurred after 4 weeks. Maximum lipid and protein were produced by saltless treatment and two concentrations of NaCl 25, 200 mM), respectively. Highest activity of peroxidase were seen at 200 mM NaCl but unlike the control with increasing NaCl concentration, polyphenoloxidase activity declined.
