مطالعه آنزيم پلي گالاكتوروناز قارچ (58 FUSARIUM OXYSPORUM )F عامل بيماري بوته زردي

Study of polygalacturonase of Fusarium oxysporum (F58), causal agent of chickpea yellows

Fusarium wilt disease is one of the most effective factors of Chick-pea (Cicer arietinum) crop losses in the West and North-west of Iran which is caused by Fusarium oxysporum. Many phytopathogenic microorganisms, incuding Fusarium oxysporum, attack their host plant by secreting pectic enzymes which causes modification of cell-wall structure, increasing accessibility of cell-wall components for degradation by other enzymes. To study polygalacturonase enzymes from isolate F58 of Fusarium oxysporum, enzyme purification was achieved by cation exchange chromatography. Elution of the column was carried out with 1M NaCl gradient. The presence of PG enzymes was investigated by SDS-PAGE. The results showed two distinct bands , about 33 and 35 kDa in isolate F58. These two bands showed PG activity in enzyme activity staining technique. The optimum pH for enzyme activity before and after purification was determined to be about 4.5.