اثر مراحل انجماد بر چگونگي تحرك اسپرم ماهي كپور

The effects of cryopreservation on movement patterns of carp (Cyprinus carpio) spermatozoa

The present study involved the duration of motility due to different patterns of movement and the fertility of intact and cryopreserved carp (Cyprinus carpio) spermatozoa. Cryopreserved sperms were prepared in different extenders and volumes by a four - step freezing rate. Movement of intact and thawed samples were considered according to three-pattern scale (progressive, rotatory and oscillatory). With a mean carp sperm density of 27.083X109 cell/ml and motility rate of above 80%, the duration of progressive and rotatory patterns were lower in cryopreserved sperm as compared with those in intact samples, however the duration of oscillatory movement was greater in the cryopreserved samples. Thus it is evident that this protocol lowered the progressive and rotatory movements of cryopreserved sperms which are important to produce high fertilization rates.