الگوي الكتروفورز پروتيين هاي سرم در انواع مختلف سرطان مشابه به فاز حاد است

Background and purpose : The levels of serum acute phase reactant proteins change in different malignant diseases that along with other proteins could be assessed by electrophoresis. Materials and methods: The electrophoretic pattern of serum proteins of 85 patients bearing different types of neoplasia and 85 matched normal adults were comparatively studied by agarose gel electrophoresis to find out if there is specific protein pattern in cancer. Each protein fraction was analyzed quantitatively by densitonnetry. The significant differences between variables were evaluated by studentʹs t-test. Multivariate logistic regression and ROC analysis were performed using SPSS software. Results : When total protein and albumin were measured by colorimetric methods, cancer patients showed less total protein concentration (6.3 ± 1.4 g/dL vs. 7.6 ± 0.7, P<_ 0.000) and albumin (3.9 ± 0.8 g/dL vs. 4.6 ± 0.4, P< 0.000). compared with controls Electrophoretic data of serum proteins showed that, the ratio of albumin to globulin (0.92 ± 0.30 vs. 1.21 ± 0.16, P<_ 0.000), percent of the fractions albumin (%46.7 ± 8.5 vs. %54.4 - 3.5, P< 0.000) and 13-globulin (%11.6 ± 4.4 vs. %13.0 ± 1.9, P<0.001) decreased and al (%5.3 ± 2.5 vs. %2.9 ± 0.8, P<_ 0.000), a2 (%13.5 ± 4.8 vs. %l 1.3 ± 2.1, P< 0.000) and y-globulins (%23.0 ± 7.7 vs. %18.6 ± 3.1, P< 0.000) increased significantly in cancer patients compared with controls. The cancer patients also had higher leukocytes counts (7977 ± 3111 vs. 6505 ± 1566, P< 0.000) and erythrocyte sedimentation rate (35.9 ± 23.5 vs. 14.1 - 9.5, P< 0.000). Analyses of the data using multiple logistic regression also indicated that, total protein, the ratio of albumin to globulin, the fraction of alpha- I globulin and ESR were the best parameters to discriminate malignant and normal states. The proteins profile out of the normal ranges occurred in about 83% of the cancer patients and 17% of the controls. The prevalence of cancer was strongly associated with the components of serum proteins profile. The area under the ROC curves was the same for most components of serum proteins profile as about 0.80 ± 0.05. The area was 0.59 ± 0.07, P< 0.1 for WBC counts and 0.87 ± 0.06, P< 0.000 for ESR. Conclusion : The profile of serum proteins indicated high diagnostic values for descriminating between cancer patients and healthy individuals. The determination of proteins profile may be useful as an adjunct diagnosis for early detection and screening of malignancy.