ايمني درماني 1200 نفر معتاد به مورفين در ايران بوسيله واكسن مورفين

Immunotherapy in 1200 Morphine Addict in Iran With Morphine Vaccine

Newly developed compound derived from the immune system help combat morphine abuse by destroying the drug soon after it enters the bloodstream. A morphine-6-succinyl-SA (M-6-S-SA) vaccine was designed in an effort to obtain a potent, long - lasting anti-morphine immune response for the treatment of morphine abuse. The analogue incorporated a succinic anhydride linker moiety in place of hydroxy group at c-6 of the morphine framework. Then morphine 6-hemi-succinate was conjugated to serum albumin (SA) in aqueous solution in the presence of tributylamin, isobutylehlorformate and dioxan. M-6-S-SA was synthesized in three chemical steps starting from morphine sulphate and the extent of conjugation was determined by base hydrolysis of the conjugate, extraction and measurement of free morphine or with U. V absorbance. An average of 6.5 molecules of morphine were conjugated to each molecule of protein 1200 morphine addictions from separate areas of Iran vaccinated with suitable doses in 3 steps for each addiction. After vaccination 95% of addictions immunized with three doses of the vaccine, were found to be producing antibody in 60 days later as determined by Electrophoresis method, which measures binding antigen by antibody with western blotting ELISA and TLC overlayer. Necrosis, fever, shock, etc in all steps and with whole volunteers. Finally the Morphine Vaccine also recommends for prevention of morphine addiction because we obtained very good results with 100% immune and clinical response. We also found that, there would he no cross- reaction between these antibody and analgesic drugs.