اثرات اسيد ساليسيليك بر افزايش مقاومت محورهاي جنبيني زيتون تلخ MWLIA AZEDARACH L.(

Effects of salicylic acid on enhancing the resistance of embryonic axes of Persian lilac (Melia azedarach L.) against cold and cryopreservation

In vitro conservation of germplasm is one of the best methods of plant gene pool storage. Application of this method is important for conservation of high economic and medicinal value plants and for plants that are becoming exctinct. Melia azedarach L. has high economic value, because of its high wood quality.Embryonic axes of Persian lilac (Melia azedarach L.) were encapsulated into calcium alginate capsules with sucrose (0.75M) and salicylic acid (0, 50, 200µm). Viability of encapsulated embryonic axes were studied in two conditions of cryopreservation in liquid nitrogen or preservation for 4 months at 4°c In both cases 200µm salicylic acid enhanced significantly the percentage of viability of encapsulated embryonic axes. Non-encapsulated embryonic axes did not survive after liquid nitrogen treatment. But the percentage of viability of encapsulated embryonic axes increased to 71% with 200µm SA. Also, in cold conditions, viability increased considerably from 33% in control embryonic axes to 83% in treated with 200µm SA embryonic axes. Comparison of embryonic axes viability showed that encapsulation with SA is more effective in retaining viability of embryonic axes preserved in cryopreservation than cold preservation.