بررسي اثر تنظيم كننده هاي مختلف رشد بر اندام زايي گياه كهور PROSOPIS K؟TZIANA BURKART در شيشه

Effect of different plant growth regulators on In vitro regeneration of Prosopis koelziana Burkart

Prosopis koelziana Burkart (Mimosaceac) is endemic of dry and semidry regions of Iran. Regeneration of this plant due to the hard seed coat and self incompatibility is very weak in nature- We tried to produced many plants in short time by in vitro culture. After production of sterile seedlings, different parts of seedlings were cultured on MS medium having different ranges of plant growth regulators. Node, stem and hypocotyle had almost equal potential of callus production on MS medium with BAP & NAA (2 & 0.5mg/l respectively} but in media containing (2, 4-D & Kin) and (NAA & Kin), hypocotyle was more responsive. The most effecting medium for shoot production was MS supplemented with 2, 4-D (0.5 mg/l) & Kin (1mg/l) from stem segments and for root generation was MS medium that had NAA (0.2mg/l) & Kin (0.5mg/l) from hypocotyle segments. The most number of whole plants was produced from stem segments on MS medium supplemented with BAP (1mg/l) & NAA (0-1 mg/l) 70-90% of these sterile plants survived for one month after transfer to green house conditions.