پديد آورندگان :
شيخ الاسلامي، مهيار نويسنده , , محممود اخوت ، مترجم ,
كليدواژه :
قارچ , تنوع ژنتيكي , genetic diversity , چغندرقند , Powdery mildew , Iranian Populations , RDNA-RFLP روش , rDNA-RFLP , سفيدك سطحي , Sugar beet
چكيده لاتين :
A total of 105 isolates of Erysiphe betae from different regions of Iran were investigated. For probable polymorphism of ITS-5.8S regions using PCR-based RFLP, the mentioned fragments were amplified via PCR by ITS1 and ITS4 primers and subjected to 1.5% agarose gel electrophoresis. PCR products were digested by HaeIII, AIuI, TaqI, HindIII, CfoI, EcoRI, Mspl, SacI and Rsal REs and the digestion products were resolved on 7.5% polyacrylamide. PCR product of all isolates was only one 645 bp fragment. Among nine REs tested AIuI, Cfol, Mspl and HaeIII had recognition sequences on PCR products. Because of unstability, results for AIuI were ignored.Digestion products for CfoI were 280, 185 and 180bp, for Mspl, 325, 160, 120 and 40bp and those of HaeIII, 325, 180, 75, 40 and 30bp fragments for all isolates. Electrophoretic patterns of PCR products and digested products were completely similar,thus no polymorphism was discernible between 105 isolates. Based on the results,Iranian populations of E.betae seems to be highly uniform.
