بررسي ارتباط بين پلي مرفيسم ژن اينترفرون گاما با ديابت نوع 1 در بيماران مراجعه كننده به مركز تحقيقات غدد درون ريز و متابوليسم در نيم سال اول 82

POLYMORPHISM IN IFN GAMMA GENE HAS A NEGATIVE ASSOCIATION WITH TYPE I DIABETES MELLITUS IN IRANIAN PATIENTS

Background: Type 1A Diabetes Mellitus (TIDM) is a chronic and progressive auto- immune disorder resulting from immune mediated destruction of Langerhans islet beta cells. The etiology of TIDM like the other autoimmune diseases is unknown and many factors are involved, Both humeral and cell-mediated immunity have a critical role in TIDM pathogenesis. The cytokines, the immunomodulatory peptides, are responsible for the immune cell recruitment and producing auto-antibodies by the immune effector cells. To evaluate the role of cytokines in sensitivity or resistance to TIDM, we have employed IFN gamma to determine their gene polymorphisms and their association with TIDM. Methods: 30 patient suffering from TIDM and 40 normal control were studied simultaneously .PCR technique was used to characterize the polymorphisms of cytokine. Salting out method was performed for DNA isolation .The polymorphosime of IFN gamma gene was determined on position UTR+5664ʹ5.The PCR products were evaluated by Gel Electerophoresis Technique. Results: There was a significant difference between patient and control group in TT allele IFN gamma gene: p<0.05, RR: 0.39(0.22