معرفي ژن هاي پروتيين ريبوزومي )RPL3( L3 در گندم و بررسي نقش احتمالي آنها در القاء مقاومت به بلايت فوزاريومي

RELATIVE RESISTANCE OF SELECTED ALMOND CULTIVARS TO THE CAUSAL AGENT OF RED LEAF BLOTCH DISEASE, IN CHAHAR MAHAL-VA-BAKHTIARI PROVINCE

In order to investigate the role of ribosomal protein L3 (RPL3) in inducing resistance against the mycotoxin of Fusarium graminearum, deoxynivalenol (DON) in wheat, different alleles of RPL3 gene were identified and sequenced. The RPL3 gene family in wheat consists of six genes, RPL3 A and RPL3 B loci, each having three homologs. Wheat RPL3 ESTs were obtained using yeast and rice RPL3 sequence information. We developed gene-specific primers and cloned genomic and cDNA fragments of RPL3 homologs from sensitive and resistant wheat genotypes. Sequence analysis showed a very high similarity among the homologs, while none of the expected amino acid differences related to DON-resistance were found in naturally existing alleles among the genotypes. SNP-based markers were developed which can be exploited in gene mapping and back cross selection programs. The available sequences will provide more in-depth knowledge and understanding for developing effective and durable resistance against Fusarium head blight in wheat, which is not easily overcome by the pathogen.