بررسي و مطالعه پاسخ آنزيمي قسمتهاي مختلف درختان به تيمارهاي مختلف دمايي

Enzymatic response of different parts of trees against temperature treatments

Quantitative and qualitative alternations of peroxidase and amylase aftertemperature treatments (-20, 4, 27, 50ʹ c) were determined and compared with controls (sampling at 12ʹ c). Quantitative and qualitative studies were done with spectrophotometric and gel electrophoresis methods, respectively. Investigation indicated that: 1) Isoenzymatic patterns of branch, leaf and seed werenot same in sampling time. 2) Qualitative atlernations of isoenzyme in seeds is more than branches and leaves after temperature treatment. 3) Enzyme activity of seed branches and leaves wasnot same. 4) Peroxidase and amylase activity of branch is very more than seed and leaf. 5) Results indicated production of a specific enzyme band in branch, leaf and seed after frost stress. This investigation can be used as a model for monitoring.