مطالعه توزيع داخل سلولي اينديوم در هپاتوسيتهاي موش صحرايي

Indium is a toxic element belonging to group Illa. This element and itʹs radioisotopes (111Ln, 113Ln) often are used in medical sciences, including monitoring of health in workers of atomic industries, treatment and imaging of tumor and infections and also imaging of genes expression. Therefore in this study we tried to measure the concentration of indium in different parts of the hepatocytes. After measurements of LDso, 0.35 mg/Kg indium as InCl3 was injected(I.P) daily to male rat for 60 days. Then the animals were scarified and the concentration of indium was determined in serum and homogenate tissue of liver, kidney, brain and also in nucleus, mitochondrial, lysosomal and postlysosomal fractions of hepatocytes using flamless atomic absorption. Results of this study showed that LD50 for indium ion was approximately 4.2 mg per Kg of rat weight. Chronic injection of such metal ion showed that indium was distributed in these tissues as kidney>liver > brain. Itʹs intracellular distribution in hepatocytes was in mitochondrial> nucleus> post- lysosomal> lysosomal respectively. Results obtained from this study indicated that indium mainly stored in the kidney, liver and mitochondrial, nucleus of hepatocytes.