مقايسه كارآيي روش گاز كروماتوگرافي مايع با روش مولكولي تخليص پروتيينهاي ساختماني در تشخيص و تايپينگ باكتريهاي بي هوازي

Anaerobic bacteria produce volatile and non-volatile fatty acids in enriched media containing glucose (FAB) in anaerobic conditions. The production of these acids by mentioned organisms could be used for identification purposes. On the other hands, the extraction of whole-cell proteins of strains have been introduced as a reliable technique for identification and typing of microorganisms. The aim of this study was, the comparison of efficacy of GLC method with extraction of structural proteins techniques (SDS-PAGE) in identification and typing of reference strains and clinical isolates. In this study 62 clinical strains isolated from patients and 4 reference strains obtained from collection inculiding bacteroides fragilis, mobiloncus crutisii, prevotella bivia and porphyromonas gingivalis were tested by two methods. Clinical strains isolated from patients with periodontal disease and bacterial vaginosis. Volatile and non-volatile fatty acids of species were detected by gasliquid-chromatography (method of Hoidman , et al). Whole-cell proteins of strains were also detected by Taylor , et a1. method. Most reference strains and clinical isolates produced volatile and non-volatile fatty acids in enriched media that differentiated them on the genus level, however, this method could not discriminated them on the sub-species level. The most fatty acids produced by strains were: propionic, iso-valeric, butyric, acetic, lactic, succinic and phenylacetic acids. In this study, many protein bands from 205 KDa to 37 KDa were also detected by SDS-PAGE that differentiated them well. Protein profiles of reference strains and clinical strains isolated from patients, could discriminated them on the species and sub-species levels. The species of bacteroides divided to 5 sub-species, mobiloncus to 2 sub-species, prevotella and porphyromonas each to 3 sub-species by SDS-PAGE. Our results showed that method of gas-liquid-chromatography(GLC) can be used for identification and typing of organisms on the genus level and extraction of structural proteins (SDS-PAGE) is a useful method for typing them on the species and sub-species levels, also can be use in molecular studies.