بررسي تاثير مسموميت مزمن با سرب بر تغييرات فراساختماني ارگانل هاي سيتوپلاسمي نوتروفيل طحال جنين موش

Ultrastructural Study of Cytoplasmic Organelles of Neutrophils of Fetal Rat Spleen Following Chronic Lead Intoxication

Background: Lead is one of the heavy metals that may have adverse effects on hexnatopoisis and immune system. Spleen is an organ that is involves in both the hematopoiesis and immunity. In this study, uitrastaucture and pattern of cytoplasmic granules of neutrophils in fetal rat spleen were studied following chronic lead intoxication in 2002. Materials and Methods: 30 female and 6 male Sprague Dawley rats were chosen by simple random sampling. Following copulation and vaginal plug observation, expectant rats were classified as experimental and control groups. From the first day of pregnancy, both groups received a solution containing 0.13% lead acetate and distilled water respectively. After delivery, 10 newborns from each group were chosen by systematic random sampling. The spleen of the newborn rats were fixed in a solution of 2% glutaraldehyde. After that, specimens were studied by a transmission electron microscope. Results: The ultrastructural changes in neutrophils included irregular nuclei with deep invagination, plasma membrane pockets, presence of vacuoles with a heterogeneous materials and plenty of rough endoplasmic reticulum cisternae. No differences in the mitochondrial morphology and pattern of cytoplasmic granule (primary granules with electron dense appearance and specific or secondary granules with less electron density and heterogeneous appearance) were seen in both groups. Conclusion: It seems that lead affects cells by interaction with enzyme functions or accumulation in its organelles.