طرح توليد واكسن مورفين

Designing and Synthesis of Morphine Vaccine

A morphine-6-succinyl-BSA (M-6-S-BSA) hapten was designed in an effort to obtain a potent , long-lasting anti-morphine immune response for the treatment of morphine abuse . The analogue incorporated a succinic anhydride linker moiety in place of hydroxy group at C-6 of the morphine framework . Then morphine 6-hemisuccinate was conjugated to BSA in aqueous solution in the presence of water-soluble carbodi imide . M-6-S-BSA was synthesized in three chemical steps starting from morphine sulphate , and the extent of conjugation was determined by base hydrolysis of the conjugate , extraction and measurement of free morphine - An average of 6.5 molecules of morphine were conjugated to each molecule of protein . Six rats , Albino wistar strain , immunized with various doses of the conjugate , were found to be producing antibody 8 weeks later , as determined by a modification of the (NH4)2S44 method , which measures primary binding of antigen by antibody .