جستجوي آنتي باديهاي ضد آنتي ژنها گروه يك HLA در سرم زنان باردار و چگونگي تغييرات تيتر آن در دوران بارداري

Screening of Anti HLA Class One Antibodies in the Sera of Pregnant Women and the Variation of Its Titer During Pregnancy

Determination of HLA antigens has an extensive application in paternity, Histocompatibilify testing, and disease suseptibility identification. A vast arrey of different HLA antisera are required for HLA typing. Such antisera can be obtained from pregnant women, transfused patients, immunized animals or hybridoma cells, but still the sera of pregnant women is one of the best sources for producing of HLA antisera. The pregnant women usually form antibodies to the foreign HLA antigens of the fetus inherited from the father. Due to the genetic variations among different races and the very high price of these antibodies it is preferable to obtain these antisera from locally pregnant women. In this study, sera of 40 primiparous and 65 multiparous pregnant Iranian women were screened monthly (for 3 times) againest their husbandʹs pure lymphocytes, using the microlymphocytotoxicity method of NIH. Among the primiparous 2 cases (5%) and among the multiparous women 9 cases (13.8%) had HLA antibodies in their sera. The titer and score of antibody were determined monthly in positive samples. The antibodies had the highest level in the 6th month of pregnancy. We concluded that the best source and the time to obtain HLA antisera are multiparous women in the 6th month of pregnancy.