تهيه آنتي ژن متابوليك آسپرژيلوزيس فوميگاتوس براي تشخيص سرولوژيك آسپرژيلوزيس

Preparation of Metabolic Antigen from Aspergillus fumigatus for Serodiagnosis of Aspergillosis

Aspergillosis is an important systemic fungal infection with various clinical forms and its clinical and mycological diagnosis is difficult and compelicated but its serodiagnosis is simple and useful for some clinical forms (aspergillomma and allergic aspergillosis). This study was performed for preparing metabolic antigen and related antiserum of the most important agent of aspergillosis, "Aspergillus fumigatus", for using in serodiagnosing the disease. The fungus was isolated from the patients, and cultured in glucose peptone broth for 5 weeks. After removing the fungal mass, the secretary (metabolic) antigens was isolated and processed and injected to rabbit. Antisera were evaluated by double-. diffusion (DD). Prepared antigen was able to immunize rabbits and reacted with rabbit and patientʹs sera in immunodiffusion. Number and pattern of the bands was comparable with standard antigen and antiserum. After standardization, this antigen, it can be a useful reagent to serodiagnosis of aspergillosis by lower cost and more accesibility.