بهينه سازي توليد توكسين ديفتري با استفاده از روش تاگوچي

Optimizing of diphtheria toxin production by using of taguchi method

For large scale production of diphtheria toxin, Coryneabactrium diphtheria must be cultured in a nutrient medium, and optimum conditions. In this conditions coryneabactrium produces maximum amount of toxin, which is used for diphtheria vaccine production. For obtaining maximum yield, needed to find the optimum conditions and use a suitable statistical design to determine the effects of factors and errors. For this reason in different statistical experiment designs we used orthogonal taguchi method. For this expects the orthogonal array Lʹ16 was used for the following five factors: Concenterations of 1- N. Z Amine (gr/lit), 2- Iron (mg/lit), 3- Calcium (mg/lit), 4- phosphate solution (ml/lit), 5-Growth factors solution (ml/lit) that all of them had four levels. Optimum condition and optimum response determined after analysis of experiment results. Final results showed in the optimum condition, toxin production level increased from 1201f/ml (over 60%) and comparable to other vaccine manufacturing of 4 developed countries.