تشخيص مننژيت پنوموكوكي با استفاده از تكنيك PCR-ELISA

Diagnosis of Pneumococcal Meningitidis by PCR-ELISA

APCR-ELISA was used for semiquantification of Streptococcus pneumoniae DNA in cerbrospinal fluid (CSF) due to meningitis. The PCR-ELISA was based on universal bacterial primers from 16s ribosomal RNA gene and specific probes for detection, identification and semiquantification. Only one specimen out of 45 control group specimens was positive and the optical density of the PCR-ELISA for t he rest o f them was below 1.5. All of the 38 positive CSF samples (positive culture of S. pneumoniae) were positive with the optical density of greater than 2.0(ranging 2.0 to 3.8). The sensitivity of the universal bacterial primers for S. pneumoniae was 5*103 CFU/ml. The clinical sensitivity and specificity for the test were 100% and 97.7% respectively.