القاء ساختارهاي شبه جنيني در كشت سباك ژيوتيپ هاي ذرت ZEA MAYS L

Induction of embryo-like structures in maize (Zea mays L.) genotypes via anther culture

In this study, in order to produce haploid plants for using in breeding programs and basic researches, 43 genotypes of maize (Zea mays L.) collected from the Iranian Maize Germplasm of Plant and Seed Research Improvement Institute, consisting of inbred lines, single and three-way cross hybrids were evaluated for their androgenetic response, using anther culture technique. Anthers having microspores at late-uninucleate of developmental stage were inoculated on different solid, semi-solid and liquid media of IMSS, N6, YP, YPm and CI and were incubated in darkness for 4-6 weeks at 27°c. Embryo-Like Structures (ELSs) were obtained from genotypes DH5xDH7, ETH-M82, TWC605, SC709, A188, S61, K74/1 and LA12 (sweet corn). Some of which produced calli. The frequency of ELSs in the responsive genotypes varied from 0 to 12.6% ; the genotypes DH5xDH7, ETH-M82 and S61 showed the highest response with 12.6, 4.5 and 2 ELSs respectively at 100 cultured anthers. Potential of androgenic responses that identified in some of genotypes in this research is useful for breeding maize programes .