بررسي تغييرات بافت شناسي واژن گاوميش در مراحل مختلف آبستني

Histological changes of the vaginal wall during different stages of pregnancy in buffaloes

Objective: To compare histological changes of the vaginal wall during non-pregnancy period and different stages of pregnancy in buffaloes. Design: Observational study. Animals: Fifty nine specimens from non-pregnant and pregnant buffaloes in different stages of pregnancy. Procedures: After preparation of specimens in the lab anatomy. They were stained by the method of hematoxilin - cosine and four special methods of Verhoof, Toluidine blue, Pantin and Veigerts Iron.The number of cell layer, type of epithelial cells, vascular activities, distribution of plasma cells, elastic fibers and collagen in the mucosa and submucosa were studied. Statistical analysis: One way ANOVA, Duncanʹs multiple range test. Results: During different stages of pregnancy, no significant difference in the number of cell layers and cell type of epithelium was observed. From the second month of gestation, the secretory cells were seen between the epithelial cells which increased significantly to maximum number in the fifth month (P