بررسي اثر سن و ژنوتيپ بر نحوه تگثير گيلاس وحشي از طريق كشت درون شيشه اي

In vitro Propagation of mulbery (Morus alba) Through nodal Segments

The effects of age and growth phase on micropropagation of Prunus avium was investigated. Axillary and apical buds of 4 genotypes of Prunus avium collected in winter from north forests of Iran and cultured on DKW medium supplemented with different concentrations of BA and IBA. All of the trees were in adult phase at approximate ages of 45, 100, 100 and 150 years. Shoot proliferation and elongation, vitrification and rooting were investigated.Genotype of the trees strongly influenced shooting and rooting ability and vitrification of the explants, while age of the plants did not play an important role. The best hormone concentration for shooting was 1 to 2 mg/L BA. The oldest tree showed the highest multiplication rate. Lower concentrations of BA were more suitable for shoot elongation. Genotype 3 (100-year-old tree) showed the highest rooting percentage. Genotype 4 showed the lowest percentage of vitrification and the lowest number of vitrified shoot was observed on DKW medium.Although there are significant differences between different genotypes but it seems that this propagation method can be used for all different genotypes of adult and juvenile Prunus avium plants.