تاثير عصاره ي الكلي زيره سبز بر ميزان هورمون تستوسترون و اثرات ضدباروري آن در موش هاي صحرايي نر بالغ نژاد ويستار

The Effect of Cuminum Cyminum Alcoholic Extract on Serum Testosterone Level and Its Contraceptive Effects on Adult Male Rats

Background and Objectives: One of the complications arising from the consumption of Cuminum Cyminum is reproductive system disorders in humans and animals. Since there has been no comprehensive research on the effect of this herbal medicine on spermatogenesis and production of testosterone in males, the present study was carried out with the aim of determining the effect of Cuminum Cyminum alcoholic extract on sperrnatogensis and testosterone production in adult male wistar rats in Kazeroon in 2004. Materials and Methods: In this experimental research 50 male wistar rats were divided into 5 groups of 10. Following the bioassay LD50 (lethal dose) of the compound was determined to be 120 mg/kg and Concentrations of 30,60 and 100 mg/kg as sublethal doses were injected into peritoneum every other day for 21 days in three treatment groups. The two remaining groups received the solvent (distilled water) and no compound. The results were analysed through one-way analysis of variance and t-test. ;Results: The results showed that 100 mg/kg of Cuminum Cyminum alcoholic extract reduced serum testostrone level by 71.5% (P=0.05), while it had no significant effect on serum FSH and LH levels. Histological investigations of testis showed a decline of 19.7%, 48.3% and 89.5% sperm cells in doses 30, 60 and 100 mg/kg respectively. However, no adverse effect was observed owing to the injection. Conclusion: According to the research results it can be stated that Cuminum Cyminum alcoholic extract at 100 mg/kg of rats has probably caused the weakened function and reduced testosterone level and spermatogenesis through direct effect on testicular tissues. Research concerning the ultra structure studies of testis and the effect of the plant on hormone level in hypothalamus and fetus growth is recommended.