شناسايي و تعيين تنوع ژنتيكي كلن هاي گونه هاي مختلف صنوبر با استفاده از نشانگر ميكروساتلايت

Identity and genetic diversity of populus species clones using microsatellite marker

Identification of poplar clones isnot easily possible using morphological attributes. This approach however, does not render a proper differentiation. In order to recognize the clones especially on early growth stages, molecular markers based on DNA polymorphism are suitable. In this study, 12 clones from 4 species of Populus belonging to different area of Iran, as well as 86 clones from 4 species belonging to populations of Netherlands, were investigated for screening genetic diversity and their differentiation. For using microsatellite markers, following DNA extraction from young and fresh leaves, Polymerase Chain Reaction (PCR) conditions were optimized and conducted with 7 primer pairs to amplify Simple Sequences Repeats (SSR). The PCR products were visualized using agarose gel electrophoresis and detected by staining with ethidium bromide. For demonstration of polymorphism among clones, PCR products were visualized using polyacrylamid gels electrophoresis and detected by silver staining. The data were analyzed by pop Gene software. Results showed that, the number of alleles at the SSR loci ranged from 9 to 13, with an average of 11.1 I alleles per locus. The observed heterozygosity ranged from 0.13 to 0.98, with average of 0.71 per locus. Furthermore, hybrid poplar clones have shown most heterozygosity among the poplar species. As well as poplar species and their clones has identity according to systematic classification. In this regard hybrid clones has shown most similarity with P. nigra clones. Meanwhile, similarity and genetic distance between populations has shown by a dendrogram.