بررسي ميزان تركيب هايپريسين در گونه هاي گل راعي

Extraction and determination of hypericin from plants, H. perforatum and H. scabrum

Hypericum perforatum L. (St. Johnʹs Wort) is being used and cultivated as a medicinial plant. The importance of this plant is, in particular due to the content of hypericin and its derivatives. It has recently been found that hypericin is effective against certain viruses, antineoplastic activity, protein kinase and based on the formation of singlet oxygen upon exposure to the visible light. Hypericin and its derivatives are accumulated in the multicellular nodules, around the leaf margin and around the margins of petals. Also formation of hypericins in species varies significantly depending on the origin of the plants. After collection and dried flowers of plants H. perforatum and H, scabrum from different area (Hamedan and Noshahre) wear powdered and extracted repeatedly with hot ethanol and petrol ether by soxhlet. The Hypericin content of plants are determined by uv. spectrophotometry. A significant variability with in the different Species were calculated, 1692 ppm and different area were 335 ppm.