هيستولوژي پوست و هيستومورفومتري مقايسه اي فوليكول هاي موي اوليه و ثانويه آن در مناطق گردن ، اندام خلقي و شكم ميش هاي بالغ ماكويي در فصل پاييز

Histology of skin and comparative histomorphometry of the primary and secondary hair follicles in the neck, forelimb, hindlimb and abdominal regions of Makoyee ewes in Autumn

For this purpose the skin specimens were collected from neck, forelimb, hindlimb and abdominal region from 30 apparently healthy and adult Makoyee ewes and were preserved in 10% neutral buffered formaldehyde solution. They were processed through. routine paraffin embedding. Sections were cut at 5-7µm and stained with hematoxylin-eosin, Verhoeff elastic and Van Gieson methods. Histological study revealed that, the stratum corneum of epidermis appears in the unevenly laminated form with loose keratin. Papillary region of the dermis was made up of loose connective tissue, whereas the reticular layer was made up of dense irregular connective tissue, hut relative uniformity was observed in collagen fiber diameters. Most of the primary and secondary hair follicles were in anagen or catagen stages, but relatively lesser amounts of them were in telogen stage too. Our observations revealed that apocrine sweat glands are distributed in all of the regions, but their intensity were more in abdominal part. The differences in mean distribution of primary hair follicles between all of the regions were not significant, whereas the differences in mean distribution of secondary hair follicles in between all of the regions were highly significant (p< 0.001). In all of the regions, there were very significant (p<0.001) difference in mean distribution of primary and secondary hair follicles. and in all of the cases the distribution of secondary follicles was higher than primary once. Relative estimate of secondary and primary follicles in the neck, forelimb, hindlimb and abdominal regions were 5.05 ± 0.80, 3.25+0.43, 2.58+0.21 and 2.81+ 0.11 respectively, thus the highest value was belonging to the neck and the lowest one to the hindlimb regions. Mean distribution of fleece stems in compound follicles of neck, forelimb, hindlimb and abdomen regions were 3.1+0.2, 2.85+0.1, 3.2+0.01 and 2.95+0.3 repectively.