تكثير POLYMYXA BETAE روي ريشه هاي مويين چغندر قند در كشت هيدروپونيك

PROPAGATION OF POLYMYXA SETAE ON HAIRY ROOT HYDROPONIC CULTURE

In order to mass reproduce P.betae, Agrobactrium rhizogenes was used to produce hairy roots from sugar beet petals. Root segments cut from sugar beet plants grown in soil containing resting spores were surface sterilized and placed next to hairy roots. One day after co-culturing hairy roots were transferred into hydroponics culture. Two weeks later, the presence of resting spores in hairy roots using inverted microscope was shown. Polymerase chain reaction using P.betae specific primers proved the presence of P. betae in the infected hairy roots. The ability of the newly formed resting spores to infect fresh hairy root was examined by co-culturing of the healthy and infected hairy root segments using the liquid media surrounding the infected hairy roots. PCR and microscopic observations were indicative of reinfecting ability.