اثرات مهاركنندگي كله سيتوكينين CCK8 در جذب گلوكز توسط انتروسيتهاي روده كوچك خوكچه هندي

INHIBITORY EFFECTS OF CHOLECYSTOKININ (CCK8) ON GLUCOSE ABSORPTION FROM THE SMALL INTESTINE IN VITRO

The gut is the source of many hormones and the gastrointestinal mucosa can be considered to be the largest endocrine organ. One of the three main groups of gastrointestinal hormones is cholecystokinin (CCK). The main effects of gastrointestinal hormones are related to digestion, absorption and movement of food in the gastrointestinal tract. The effects of cholecystokinin peptides on intestinal glucose transport were investigated by using in vitro techniques. The effects of CCK8 (the C, terminal octapeptide of (CCK33), sulfated and non - sulfated on the absorption of glucose from the guinea pig small intestine were studied using mini and large transport chambers. In small transport chambers , the paired sheets with CCK8 were studied as the experimental group while the paired sheets without CCK8 were used as control. In the large transport chambers , each sheet was used as a control . CCK8, both sulfated and non - sulfated inhibited glucose absorption by enterocytes up to 45% though the concentration of non sulfated CCK8 required for inhibition was more than the concentration of 1µg of sulfated CCK8 required for inhibition. The inhibitory effects of CCK8 on glucose absorption depended upon the cholecystokinin receptors (A & B). CCK8 (sulfated) had more affinity towards receptors as compared to CCK8 (non - sulfated) and therefore lesser concentrations were required to inhibit glucose absorption.