نقش روزنه ها در مرحله انتقال و سازگاري ريزازدياي گياهچه هاي آلوكك PRUNUS AVIUM L.

In order to examine the factors affecting the normal growth and survival of plantlets of wild cherry (Prunus avium L.) after transferring from in vitro conditions into soil, an anatomical and physiological study was carried out on leaf stomata in three conditions, in vitro and ex vitro after 2 and 4 weeks. The anatomical observation showed no significant differences between stomata of in vitro and ex vitro plantlets in shape, size and frequency. Stomata of in vitro showed malfunctioning in response to light, C02 concentrations and water deficit comparing to those transferred and acclimatized in soil after 4 weeks. The concentrations of K+, Na+ and Ca^2+ in leaves of in vitro plantlets indicated that these ions could involve in this malfunctioning. The presence of high concentration of nitrogen and sodium in leaves of in vitro cultured of wild cherry could also inactivate ABA, resulting in lack of stomata responses to environmental stresses.