بررسي اثر هيدروكورتيزون سيستميك بر تعديل علايم ناشي از قطع مرفين در موش سوري

The Effect of Peripheral Injection of Hydrocortisone on Withdrawal Syndrome in Addicted Mice

Background: Tolerance and dependency are two main problems that have limited morphine administration. There are several reports concerning the role of some of these factors in the occurrence of tolerance and dependency. However, the precise mechanism of tolerance is not known yet. It has been suggested that corticosteroid drugs may be involved in morphine-induced tolerance and dependency. The aim of this study was determination of effects of corticosteroids (Hydrocortisone) on modification of morphine-induced symptoms in physiology research center of Semnan in 2003. Materials and Methods: In this experimental study 30 male albino mice (weighing 25-30 gr) were divided into one control and two case groups. Morphine was used to produce drug dependency by marshall method. As the animals daily received three doses of morphine (50, 50, 75 mg/kg) in three consecutive days. On the fourth day 90 minutes after injection of 50 mg morphine hydrocortisone was injected into experimental groups (5 and 10 mg/kg subcutaneously) and the same volume of vehicle was injected into control group. After 20 minutes (2mg/kg) Naloxan was injected subcutaneously to cause withdrawal syndrome. To evaluate withdrawal syndrome number of jumps, weight loss (evacuation) and latency period were used and the data were analyzed by T-test. Results: The results indicated that hydrocortisone (5 and 10 mg/kg) significantly decreased withdrawal syndrome (P<0.01). Conclusion: Above findings indicated that secreted steroids from cortex of adrenal probably had important role in modification of withdrawal syndrome. In order to ascertain the function of neurotransmitter systems and other related factors more research is recommended.