بررسي آلوايمونيزاسيون در بيماران مبتلا به تالاسمي ماژور شهرستان زاهدان در سال 1380آ

Evaluation of alloimmunization in major B. thalassemic patients in Zahedan in 2001

Background and purpose: Increasing transfusion need is one of the main problems in thalassemic pathients. Alloimmunization is one of the most complicated causes of this problem which is an immunologic response to foreign antigens. According to different studios, its frequency is 57, to 217. in major B-thalassemic patients. Alloimmunization leads to decrease RBCs life span, an so, demands for increasing frequency of blood transfusion. Main aim of this research was to study frequency of alloimmunization and to determine type of involved antigen. Reffering to the obtaind data, it could help us plan proper match transfusion and appropriate medication in detected patients. Detection of correlation between alloimmunization and the parameters such as, size of liver and spleen, gender, blood groups and annual mean Hb concentration were the other purposes of this study. Materials and methods: In this study, serum of 162 B- thalassemic patients with frequent transfusion( more than 240 cc/kg/yr) regardless of gender, age and type of transfusion(with and without leukocyte filter) were studied. Alloimmunization was studied by normals saline, 227. albumin and antihuman globulin with screen 0-cell indicator methods. All of the cases under study were negative for alloimmunization. For confirmation, sera of 96 patients were sent to Tehran reference blood transfusion center, panel test was done, the results indicated no alloimmunization. Results: Inspite of accurate performance of the tests and repeatation, there was no case of alloimmunization. Conclusion: In contrast to the previous studies indicating the prevalence of alloimmanization in B- thalassemic patients as 5-21 %, but the results of this study which were confirmed by reference laboratory showed that, there was no case of alloimmanization. It can be expressed that,there is antigenic similarity between blood donnars and recipients in close consanguinity of sistan and balochestan province.