ريزازديادي رز اليزابت به روش كشت بافت در شرايط درون شيشه اي

In this research, the first maternal plants selected from Elizabeth cultivar were kept in the greenhouse for environmental treatment and control of the diseases and pests for 1-2 months. Nodal segments were selected at the 2cm size and were sterlized in 2.8% sodium hypocholoride. They were cultured in a modified MS medium containing different levels IAA, BAP and severals subcultures. Then isolated shoots were placed in different rooting culture medium. The implemented observations showed significant differences between IAA levels, BAP levels and several subcultures and reciprocal effects between them. In this experiment, MS medium (supplemented with 0.1 mg/1 IAA and 1 mg/l BAP and 3 stages of subculture) induced medium (supplemented with 0.1 mg/l IAA and 1 mg/l BAP and 3 stages of subculture) induced the highest number of new shoots. In the rooting stage camparsion between few culture medium on the rooting of the plantlets showed significant difference, and MS medium including 0.5 mg IAA produced the best roots (75% rooting). Produced plants then were transported to mixed medium sand and peat in the ratio of 1:1 and kept in greenhouse.