جداسازي و شناسايي و تعيين مقدار GINGEROL از ريزوم زنجبيل

Isolation Identification and Quantitative determination of Gingerols in ginger roots

The rhizomes of ginger were obtained from local market and then macroscopic and microscopic properties of powder evaluated. The charactristic components were similar with Zingiber officinale. Ground rhizomes were repeatedly extracted with acetone on room temprature and then combined extractes were evaporated in vacuum to afford a residue, which diluted by distilled water and extracted with petroleum ether and ethylacetate respectively. The evalutions with TLC showed that the most of active ingredients present in petroleum ether fraction. The remaining of this extract after evaporation of solvent was fractionated on silicagel column (silica gel G60 70-230 Mesh, Merck) using gradient of Petr. Ether/ EtOAc (92.5:7.5_ 90:10) and Petr. Ether/ EtoAc/CHC13 (80:15:15____55:40:5) to afford 7 main groups of fractions. Fractions 4-7 were purified by prep. TLC method and finally the [6] - gingerol and mixture of [8] and [10J gingerol were obtained. Using UV, IR, ʹH-NMR and l3C-NMR the presence of these components in ginger rhizome, dacumented. Quantitative analysis of these compounds showed that the [6]gingerol, mixture of [8], [10]- gingeroles and total amount of gingerols in the rhizome were 4.96%, 6.43% and 11.04% respectively.