شناسايي كروموزومها در سورگوم ديپلوييد SORYHUM BICOLOR L. M؟NCH به وسيله روش C-BANDING

Chromosome identification in diploid Sorghum bicolor (L). Moench by C - Banding

Chromosome banding patterns, showing number, location and size of heterochromatin bands, provide unique ways to identify individual chromosomes of a complement, to detect chromosomal abnormalities and to elucidate phylogenetic relationships among related species. This pattern can be used as the basis for detecting intraspecific chromosomal variability and interspecific genomic relationship. Howevere, such banding patterns in Sorghum bicolor (L). Moench have rarely depicted exept for Sorghum bicolor cv. Combine kafir 60. Since these chromosomes are small, they are difficult to be distinguished. In this study, c-banding technique was applied to the line IS2325 (2n=20) to determine the banding patterns of the chromosome complement. All chromosomes of the line IS2325 showed intensive centromeric c-bands. Intercalary bands were present in chromosomes, 3, 6, 7 and 10 with some variation in their position. Telomeric c-bands were indentified in both arms of chromosomes 1 and 2 and in long arm of chromosomes 4 and 5. Chromosomes 8 and 9 had only centromeric c - bands. Some c - bands were constant in showing their characteristics and some were facultative. All of the 10 chromosome pairs in the line IS2325 were identified by their c-banding patterns, along with their length and arm ratios.