بررسي تغييرات هيستولوژيك و مرفولوژيك بافت بيضه رت بالغ در دوران حاد قطع نخاع

Study of Histologic and Morphologic changes in Matured RA T testicular tissue in acute period of spinal cord transsection

Background and purpose : Spinal cord injury at caudi equine region, would cause about 10-20% of vertebral column damages, which is followed by paraplegia, and most of the patients of such cases are in the age group of 16 to 30 years. Materials and Methods : In this study histological and morphological changes in Sprague-Daweley rat testies in the first, second and third week of spinal cord transsection was evaluated. In the test group spinal cord was transsected at to level. And in the control group, skin was cut but laminectomy and spinal cord transsection was not performed. In a proposed time testies were taken out and after determining the weight and volume, fixation by Buinʹs solution was done, to be observed by light microscope. After processing the samples, the histologic changes were. determined by H&E, PAS, massonʹs trichorom and toloiudine blue staining techniques. Results : The collected data indicales the following changes in the testies of the rats in the rats in the test group-Decline in the number of germ cells in epithelium ofʹ spermatogenic act, decline in the percetage volume of spermotogenic act, declibe in the volumic percentage of interstitial space, lack of changes in the number of sertoli cells, lack of changes in leydig cells and interestial tissue components, increase in the severity of above changesas time passes. Conclusion : Generally, it can be concluded that spinal cord transsection would cause changes in the normal activity of the testicular tissue and natural role of internal and external factors. and cases disorders in the process of spermatogenesis.