مقايسه تستهاي تشخيصي و باكتريولوژيك بروسلوز در شهر اهواز

Serological and Bacteriological Studies of Brucellosis in Ahwaz

In this study a total of 450 individuals who were suspected of having brucellosis and who had referred to several clinical laboratories in Ahwaz were sampled. Two specimens from each person, a serum sample for serological tests and a whole blood sample for culture, were collected. On the basis of standard tube agglutination (STA) and Coombʹs Wright titers, the patients were divided into 3 groups: Group 1- 280 (62.3%) individuals with negative standard tube agglutination, Coombʹs Wright and blood culture. Group 2- 95 (21.1%) individuals with titers of 1:20 to 1:80 for standard tube agglutination and a negative blood culture. Group 3- 75 (16.6%) individuals with titers of 1:160 to 1:2560 for standard tube agglutination and Coombʹs Wright, 7(9.34%) individuals in this group had a positive blood culture. The 7 positive blood cultures were subjected to different biochemical tests and their sensitivty to special dyes were determined. As a result: 2 brucella abortus strains and 5 brucella melitensis strains were isolated. The most reliable laboratory test for diagnosis of brucellosis is blood culture, but brucella are fastidious and require a long time and specical conditions to grow. The serological tests for diagnosis of brucellosis are simple and fast. Therefore, both serological and blood culture procedures should be used in parallel.