تاثير كربوهيدرات ، جيبرليك اسيد، انيدول بوتيريك اسيد، فلوروگلوسينول ، نحوه قرارگيري ريزنمونه در محيط و حجم ظروف كشت در بهينه سازي تكثير درون شيشه اي پايه M.9 سيب

Effect of carbohydrate, gibberellic acid, indolebutyric acid, phloroglucinol, explant orientation and culture vessels volume on optimizing in vitro propagation of M.9 apple rootstock

In this research, with the purpose to optimize in vitro shoot proliferation of M.9, the factors of carbohydrate, gibberellic acid (GA3), indolebutyric acid (IBA), phloroglucinol (PG), explant orientation and culture vessels volume were studied. The basal medium was MS supplemented with 1.5 mg.l"1 BA and 1 mg.l"1 Kin. Shoot explants 1 cm in length were taken from in vitro samples. The results showed that sorbitol was more suitable as a carbohydrate for multiplication than sucrose. GA3 and PG had no effect on number of shoots, but affected length of shoots. Application of IBA had no effect on proliferation. Explants orientation has significant effect on proliferation and the best results were obtained with vertical orientation. Increase in vessel volume from 25 ml to 125 ml had no effect on shoots proliferation of M.9. On the basis of the results, is suggested for improving of M.9 proliferation were used from basal medium culture (MS with 1.5 mg.l1 BA, 1 mg.1-1 Kin and 8 g.l"1 agar) containing 30 g.l"1 sorbitol, 0.1 mg.l1 GA3, 162 mg.l1 PG. Also, shoots put in medium vertically.