مقايسه روش اندازه گيري پروتوپورفيرين سرم با ساير آزمونهاي آزمايشگاهي براي تشخيص كم خوني فقر آهن در اطفال

COMPARISON OF THE DIAGNOSTIC PERFORMANCE OF SERUM PROTOPORPHYRIN ASSAY WITH THAT OF TRADITIONAL TESTS OF IRON DEFICIENCY ANEMIA IN CHILDREN

Iron deficiency anemia is a significant public health problem. The elimination of iron deficiency in children has been a major concern of research workers in the past few years of those who care for in this study, we investigated the importance of serum protoporphyrin for the detection of iron deficiency anemia. The purpose of our study was to evaluate serum protoporphyrin screening in young children. 79 children between 6-11 years of age and having signs of iron deficiency anemia and low serum ferritin levels were enrolled as experimental group over a 4 year period, and 74 children between 8-12 years with no signs of iron deficiency anemia and having high serum ferritin levels were enrolled as a control group again over a 4 year interval Protoporphyrin was determined asing Shimadzu RF 5000 spectroflurophotometer. The excitation wavelength was 457 nm and the fluorescence spectra were scanned from 400-600nm. Hematocrit was performed by contrifugation of a tube. Serritin, transferin saturation, total iron binding capacity and serum iron were determined by Cecil-CE spectrophotometric method. Serum protoporphyrin method has a sensitivity of 93.3%, specificity of 91.5% and a predictive value of 68.3% and is appropriate compared to other methods used for the detection of iron deficiency anemia serum protoporphyrin concentration in iron deficiency anemia group was 4 times higher compared to that the control group and a statistically meaningful difference existed between the control and patientsʹ groups (P<0.05). The data indicate that the serum protoporphyrin value obtained by this method could be used as a screening test for iron deficiency anemia.