مطالعه تاثير سه روش متفاوت خوراندن آغور بر مقادير پروتيين تام بتاگلوبولين و گاماگلوبولين سرم خون گوساله هاي نوزاد شيري

Comparison of three methods of colostrum administration in relation to serum total protein and globulin levels in neonatal dairy calves

The experiment was carried out on 45 neonatal dairy calves (Holstein) divided into three groups and treated differently during the first two days of life according to method of colostrum administration.Treatments were: Single box and no contact with the dam. Colostrum was administrated via nipple bottle at the rate of 2.5 lit every12 hours for 48 hours (group 1), cow and calf together in a maternity pen and colostrum administrated primarily by nipple bottle at a rate of 2.5 lit one time and then by free suckling until 48 hours of age (group 2), and cow and calf together and free suckling for 48 hours with primary help for starting of suckling as soon as possible (group 3). Sampling was conducted from jugular vein of calves at 48 hours post partum and blood was transferred to plane tube. Serum was harvested by centrifugation and the levels of serum total protein, â globulin and ? globulin were measured by Biuret colorimetric method and cellulose acetate electrophoresis.Highest levels of serum total protein, â globulin and ? globulin were measured in group 1. Lower amounts of serum total protein, â globulin and ? globulin were measured in groups 3, 2 and 3, respectively. Significant differences were seen between groups for the serum total protein and ? globulin levels (p< 0.05). Duncan test suggested significant differences between groups 1 with 2 and groups 1 with 3 for serum total protein and also groups 1 with 3 for ? globulin levels (p< 0.05).